Objectives
   •
   Plasma Separation, and calculation of percentage 
   •
   Serum Separation, and calculation of percentage
   •
   Plasma Proteins separation and detection 
    Separation of Plasma and Serum from Whole Blood
    1-Plasma Separation
                Centrifuge at 3000                                                     •   Record the volume of the Whole 
                rpm
                for 10 minutes                                  Plasm                      Blood (V1)
                                                                a
                                                                                       •   Record the volume of the plasma 
                                                                Red Blood                  (V2)
                                                                Cell
                                                                                       •   Record the volume of the RBC (V3) 
                                                                                           and 
                                                                                       •   Calculate the percentage and 
                                                                                           compare it to normal
       Results
                      Component         Total Volume        Percentage (%)
                1     Whole Blood    V1=
                2        Plasma      V2=
                3         RBCs       V3=
          Separation of Plasma and Serum from Whole 
          Blood
          2-Serum Separation
                  Centrifuge at                                                              •   Record the volume of the Whole 
                  3000 rpm
                  for 10                                                                         Blood (V1)
                  minutes                                                                    •   Record the volume of the Serum 
                                                                                                 (V2)
                                                                                             •   Record the volume of the RBC 
                                                                                                 (V3) and 
                                                                                             •   Calculate the percentage and 
                                                                                                 compare it to normal
       Results
                      Component         Total Volume        Percentage (%)
                1      Whole Blood    V1=
                2         Serum       V2=
                3         RBCs        V3=